antibody against connective tissue growth factor ctgf (Santa Cruz Biotechnology)
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Antibody Against Connective Tissue Growth Factor Ctgf, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 773 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ctgf/CTGF+Antibody/pm42785080-52-0-11
Average 96 stars, based on 773 article reviews
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Blocking Assay:Article Title: Hepatocyte Growth Factor Confers Trabecular Meshwork Stem Cell Resilience and Paracrine Protection of Trabecular Meshwork Cells in Glaucoma. Article Snippet: Equal protein amounts (12 μg) were separated on 8% to 16% Tris-glycine gels (Invitrogen, Carlsbad, CA, USA) and transferred to polyvinylidene difluoride membranes (MilliporeSigma). .. Membranes were blocked with EveryBlot Blocking Buffer (Bio-Rad, Hercules, CA, USA) for 5 minutes at room temperature and incubated overnight at 4°C with primary antibodies against CHI3L1 (R&D Systems, 1:500), MYOC (a kind gift from Dr. W. Daniel Stamer, Duke University, 1:3000), OCT4 (Cell Signaling Technology, Danvers, MA, USA; 1:1000), Article Title: Hepatocyte Growth Factor Confers Trabecular Meshwork Stem Cell Resilience and Paracrine Protection of Trabecular Meshwork Cells in Glaucoma. Article Snippet: Cells were washed with PBS, fixed in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) for 15 minutes, and permeabilized with 0.2% Triton X-100 (MilliporeSigma) for 10 minutes. .. After blocking with 1% bovine serum albumin (MilliporeSigma) for 30 minutes, samples were incubated overnight at 4°C with primary antibodies against CHI3L1 (R&D Systems, 1:100), MYOC (a gift from Dr. Stamer, 1:300), OCT4 (Cell Signaling Technology, 1:200), Ki67 (Abcam, 1:200), 8F1-3 (a kind gift from Dr. Nirmala SundarRaj, University of Pittsburgh, 1:200), Article Title: Hepatocyte Growth Factor Confers Trabecular Meshwork Stem Cell Resilience and Paracrine Protection of Trabecular Meshwork Cells in Glaucoma Article Snippet: Cells were washed with PBS, fixed in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) for 15 minutes, and permeabilized with 0.2% Triton X-100 (MilliporeSigma) for 10 minutes. .. After blocking with 1% bovine serum albumin (MilliporeSigma) for 30 minutes, samples were incubated overnight at 4°C with primary antibodies against CHI3L1 (R&D Systems, 1:100), MYOC (a gift from Dr. Stamer, 1:300), OCT4 (Cell Signaling Technology, 1:200), Ki67 (Abcam, 1:200), 8F1-3 (a kind gift from Dr. Nirmala SundarRaj, University of Pittsburgh, 1:200), Article Title: Hepatocyte Growth Factor Confers Trabecular Meshwork Stem Cell Resilience and Paracrine Protection of Trabecular Meshwork Cells in Glaucoma Article Snippet: Equal protein amounts (12 μg) were separated on 8% to 16% Tris-glycine gels (Invitrogen, Carlsbad, CA, USA) and transferred to polyvinylidene difluoride membranes (MilliporeSigma). .. Membranes were blocked with EveryBlot Blocking Buffer (Bio-Rad, Hercules, CA, USA) for 5 minutes at room temperature and incubated overnight at 4°C with primary antibodies against CHI3L1 (R&D Systems, 1:500), MYOC (a kind gift from Dr. W. Daniel Stamer, Duke University, 1:3000), OCT4 (Cell Signaling Technology, Danvers, MA, USA; 1:1000), Incubation:Article Title: Hepatocyte Growth Factor Confers Trabecular Meshwork Stem Cell Resilience and Paracrine Protection of Trabecular Meshwork Cells in Glaucoma. Article Snippet: Equal protein amounts (12 μg) were separated on 8% to 16% Tris-glycine gels (Invitrogen, Carlsbad, CA, USA) and transferred to polyvinylidene difluoride membranes (MilliporeSigma). .. Membranes were blocked with EveryBlot Blocking Buffer (Bio-Rad, Hercules, CA, USA) for 5 minutes at room temperature and incubated overnight at 4°C with primary antibodies against CHI3L1 (R&D Systems, 1:500), MYOC (a kind gift from Dr. W. Daniel Stamer, Duke University, 1:3000), OCT4 (Cell Signaling Technology, Danvers, MA, USA; 1:1000), Article Title: Hepatocyte Growth Factor Confers Trabecular Meshwork Stem Cell Resilience and Paracrine Protection of Trabecular Meshwork Cells in Glaucoma. Article Snippet: Cells were washed with PBS, fixed in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) for 15 minutes, and permeabilized with 0.2% Triton X-100 (MilliporeSigma) for 10 minutes. .. After blocking with 1% bovine serum albumin (MilliporeSigma) for 30 minutes, samples were incubated overnight at 4°C with primary antibodies against CHI3L1 (R&D Systems, 1:100), MYOC (a gift from Dr. Stamer, 1:300), OCT4 (Cell Signaling Technology, 1:200), Ki67 (Abcam, 1:200), 8F1-3 (a kind gift from Dr. Nirmala SundarRaj, University of Pittsburgh, 1:200), Article Title: Hepatocyte Growth Factor Confers Trabecular Meshwork Stem Cell Resilience and Paracrine Protection of Trabecular Meshwork Cells in Glaucoma Article Snippet: Cells were washed with PBS, fixed in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) for 15 minutes, and permeabilized with 0.2% Triton X-100 (MilliporeSigma) for 10 minutes. .. After blocking with 1% bovine serum albumin (MilliporeSigma) for 30 minutes, samples were incubated overnight at 4°C with primary antibodies against CHI3L1 (R&D Systems, 1:100), MYOC (a gift from Dr. Stamer, 1:300), OCT4 (Cell Signaling Technology, 1:200), Ki67 (Abcam, 1:200), 8F1-3 (a kind gift from Dr. Nirmala SundarRaj, University of Pittsburgh, 1:200), Article Title: Hepatocyte Growth Factor Confers Trabecular Meshwork Stem Cell Resilience and Paracrine Protection of Trabecular Meshwork Cells in Glaucoma Article Snippet: Equal protein amounts (12 μg) were separated on 8% to 16% Tris-glycine gels (Invitrogen, Carlsbad, CA, USA) and transferred to polyvinylidene difluoride membranes (MilliporeSigma). .. Membranes were blocked with EveryBlot Blocking Buffer (Bio-Rad, Hercules, CA, USA) for 5 minutes at room temperature and incubated overnight at 4°C with primary antibodies against CHI3L1 (R&D Systems, 1:500), MYOC (a kind gift from Dr. W. Daniel Stamer, Duke University, 1:3000), OCT4 (Cell Signaling Technology, Danvers, MA, USA; 1:1000), Immunofluorescence:Article Title: Exploiting TGF-β-mediated Stromal Programming in Homologous Recombination-Deficient Pancreatic Cancer Article Snippet: F-actin was stained with phalloidin-Atto565 (1:500-1:1,000; Sigma-Aldrich). .. For immunofluorescence, primary antibodies against ACTN4 (1:250; clone EPR2533( ); Abcam ab108198), αSMA (1:500-1:1,000; clone 1A4; Abcam ab7817), B220 (1:25; clone RA-6B2; Santa Cruz Biotechnology sc-19597), CD11b (1:100; clone M1/70; Cell Signaling 46512), CDH1 (1:250; clone 24E10; Cell Signaling 3195), CDH2 (1:200; clone D4R1H; Cell Signaling 13116), CK19 (1:35; clone TROMA-III; DSHB 2133570), Control:Article Title: Silencing KMT2A with siRNA induces apoptosis and cell cycle arrest in high-grade serous ovarian carcinoma cells by modulating GOF p53-dependent pathways, highlighting its potential as a therapeutic target. Article Snippet: 1 Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz 5166616471, Iran 2 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Abstract High-grade serous ovarian carcinoma (HGSOC) is the deadliest gynecological cancer, often characterized by TP53 mutations that result in gain-of-function (GOF) p53, contributing to the aggressive nature of the disease.. Recent studies demonstrated that GOF p53 drives cancer progression by recruiting epigenetic regulators that activate the expression of oncogenic genes.. One such regulator is lysine methyltransferase 2 A (KMT2A), an enzyme that modulates gene expression by methylating histone H3 at lysine 4. other:Article Title: Nucleus-translocated glucokinase functions as a protein kinase to phosphorylate TAZ and promote tumour growth Article Snippet: Normal mouse IgG (sc-2025), normal rabbit IgG (sc-2027), GST (sc-138), Importin α1 (sc-55538), c-SRC (sc-8056), CK2α (sc373894), PIN1 (sc-46660), TAZ (sc-518026), CTGF (sc-101586) and |
